Incucyte® Live-Cell Analysis Devices

The goal of this content is to serve as a resource for anyone wanting to learn more about long-term, live-cell imaging. Explore the resources below to learn more about how live-cell analysis outperforms other methods. Generate data rich information from every sample with fluorescent reagent combinations – compatible with a variety of cell culture models and applications Spend more time investigating and less time troubleshooting with lab-tested protocols and purpose-built, intuitive software Unlock 3D biology with ease using intuitive software and automated processes to acquire and analyze complex biology
To ensure accurate representation of cellular dynamics, it's important to optimize acquisition speed, resolution, and time intervals based on the specific biological phenomena being studied. However, over-collection of images can lead to excess data, increasing processing time and storage demands without necessarily enhancing biological insight. The ability to quickly capture images is crucial for studying fast kinetics in cellular activities.

Compare Incucyte® Live-Cell Analysis Systems

The integration of 3D cell culture further enhances this approach by mimicking the structural complexity of living tissues, offering deeper insights into development, disease, and therapeutic response. Live-cell imaging has become essential for studying the dynamic biology of living cells. Make new discoveries with a wide range of applications  — from complex immune-tumor cell interactions, synaptic activity in neuronal co-cultures, metabolism in cancer cells, and much more.
These systems typically do not sit within a tissue culture incubator and so are limited in terms of environmental stability and long-term studies. As such, it becomes easier to target internal cellular structures with dyes and antibodies. The fixation process kills the cells, which makes the outside cell membrane more permeable than living cells. While cellular composition is compromised, larger structures such as proteins, organelles and DNA are conserved. Fixed cells have been preserved by a fixation step that “locks” the cell in place.

Acquire and View Images Over Time

Maintain your cell health with non-perturbing image-based analysis and proprietary reagent formulations. The Incucyte® itself is not an incubator, but rather sits within a standard tissue culture incubator so cells are maintained in a physiologically relevant environment. Learn about the benefits of AI-driven image analysis and label-free cytotoxicity assays Real-time live-cell analysis is redefining the possibilities and workflows of cell biology. Furthermore, the applications enable analysis of a wide range of phenotypic cell-based assays. With the Incucyte® S3 Live-Cell Analysis System, take your research further with automatic acquisition and analysis of cells in a physiologically relevant environment.

AI-Driven, Label-Free Quantification of Cell Viability

Automated systems in live-cell analysis streamline image acquisition and processing, enabling efficient, accurate, and reproducible results. Fluorescence microscopy systems are designed to acquire images at high speeds, enabling researchers to observe rapid changes and dynamic processes in real-time. By tagging specific molecules with fluorescent markers, researchers can track their movement, concentration, and interactions within the cell, providing valuable data for understanding cellular mechanisms. The ability to selectively label and visualize specific molecules provides valuable insights into cellular processes that would otherwise be invisible. Efficient data management systems and advanced analytical tools are essential to handle this influx of information, enabling researchers to extract meaningful insights without being overwhelmed by the data.

Minimization of Focusing Errors and Image Artifacts

Request a complimentary sample of our ready-to-use transfection reagents for life science research involving most mammalian cell lines. Accelerate your next discovery with Incucyte’s® suite of live-cell applications. Reagents have been optimized for each application allowing easy assay preparation and intuitive software that guides the user through each step. These systems again lack proper environmental controls which will impact long-term studies. With increasing technical advancements comes increased complexity and costs; high-resolution microscopes often require extensive training and access is often limited. The timing of adjunct studies such as analysis of metabolites or secreted proteins in supernatants can also be guided.

• With continuous live-cell imaging and analysis, it is straightforward to temporally track network parameters and use the real-time data to judge when best to initiate treatment regimes.
• Spend more time investigating and less time troubleshooting with lab-tested protocols and purpose-built, intuitive software
• Fluorescence microscopy allows for precise quantitative measurements of various cellular processes.
• Incucyte® S3 shares the same highest maximum throughput as the Incucyte® SX5 but supports only the phase channel as well as green and red fluorescence.
• Generate data rich information from every sample with fluorescent reagent combinations – compatible with a variety of cell culture models and applications
• The volume of data generated during long-term imaging experiments can be substantial, posing challenges in storage, processing, and analysis.
• Live-cell imaging of T cells engineered to target and kill GFP labelled tumor cells.

Proper image optimization reduces noise and enhances the signal, making it easier to extract quantitative information from the images. This optimization process is crucial for obtaining clear and accurate images that can be reliably analyzed. A well-defined experimental plan ensures that the study is focused and that the data collected is relevant and meaningful.

• These software solutions often include advanced features such as real-time image processing, customizable analysis, and detailed reporting tools.
• We provide remote support, additional training and other servic…
• Cells are dynamic in nature, so it is important to have the ability to image in real time to gain access to deeper biological insights.
• Power your research with the Incucyte® platform of real-time live cell analyzers.
• These systems typically do not sit within a tissue culture incubator and so are limited in terms of environmental stability and long-term studies.
• To ensure the best possible image quality, researchers must optimize various settings such as exposure time, focus, and illumination intensity.

Imaging fixed cells allows high-resolution imaging of sub-cellular structures. See how your peers are using live-cell imaging and analysis Live-cell imaging continues this legacy by providing deeper insights into cellular processes, leading to advancements goatz casino no deposit bonus in medical research and biotechnology.
The integrated software simplifies data analysis to accelerate response time, including the creation of publication-quality graphs and charts. Capture high-resolution fluorescence and brightfield images, recording data in real-time over hours, days, or weeks. Cells are dynamic in nature, so it is important to have the ability to image in real time to gain access to deeper biological insights. Traditional end-point assays only provide single measurement of cellular events. Live-cell imaging and analysis should be used when studying any area of cell therapeutics.

The content of our website is always available in English and partly in other languages. See how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data. Incucyte® S3 shares the same highest maximum throughput as the Incucyte® SX5 but supports only the phase channel as well as green and red fluorescence. For the Incucyte® S3, an incubator larger than 200L is recommended.